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Proteins are the key effectors of any living system and are largely responsible for the functioning of a cell. Intricate cell signaling and molecular triggers are dependent on interactions involving proteins at the cellular level. It is due to this very reason that, in an age where clinical biology is thriving to make an impact in global health-care and biomedical diagnostics, there has been a surge of interest in the area of Interactomics. Interactomics essentially involves the study of interactions between biomolecules, particularly proteins and the consequences of those interactions in a biosystem.
This course of interactomics focuses on two powerful platforms:
1. Protein microarrays and
2. Label free biosensors involving Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI).
While the former is a high-throughput tool for discovery-based interactomics, the later is proving itself to be an instrumental resource for validation based studies. This course details the overlapping domains of proteomics and interactomics, which form the focus of researchers making an impact in the clinical biomarker and pharmaceutical arena across the globe. Students would benefit hugely from this unique course-model designed for budding scientists as well as seasoned researchers new to this area of research
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Week 1:
Lecture 1 : Introduction to interactomics
Lecture 2 : An overview of label-free technologies
Lecture 3 : An overview of surface plasmon resonance (SPR)
Lecture 4 : An overview of surface plasmon resonance imaging
Lecture 5 : Basics of SPR: Surface chemistry
Week 2:
Lecture 6 : Basics of SPR: Experimental design
Lecture 7 : Protein immobilization for protein-protein interaction studies
Lecture 8 : Protein-protein interaction study: Binding analysis
Lecture 9 : Protein-protein interaction study: Kinetic analysis
Lecture 10 : Protein-small molecule interaction study: Immobilization & binding analysis
Week 3:
Lecture 11 : Protein-small molecule interaction study: Kinetic analysis
Lecture 12 : SPR: Interactive Session-I
Lecture 13 : SPR: Interactive Session-II
Lecture 14 : An overview of ellipsometry and interferometry techniques
Lecture 15 : An introduction to BioLayer Interferometry (BLI) and its applications in protein research
Week 4:
Lecture 16 : Kinetic analysis of protein-protein interaction using BLI
Lecture 17 : Label-free quantification of proteins using BLI
Lecture 18 : Diffraction-based biosensors I
Lecture 19 : Diffraction-based biosensors II
Lecture 20 : Nanotechniques in proteomics I
Lecture 21 : Nanotechniques in proteomics II
Week 5:
Lecture 22 : High throughputplatforms of interactomics: Protein arrays
Lecture 23 : Conventional label based detection techniques for Protein microarrays
Lecture 24 : Novel detection techniques for Protein microarrays
Lecture 25 : Recombinational cloning and its application for Protein microarrays
Lecture 26 : An introduction to Cell-free protein synthesis
Week 6:
Lecture 27 : Cell-free synthesis based protein microarrays: PISA and NAPPA
Lecture 28 : Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays
Lecture 29 : Digging deeper into NAPPA: Basic Workflow
Lecture 30 : Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment
Lecture 31 : Application of cell free expression protein microarrays in biomarker discovery
Week 7:
Lecture 32 : Application of cell free expression protein microarrays in immunological studies
Lecture 33 : Basics of microarray image scanning
Lecture 34 : Software for Image scanning and data processing
Lecture 35 : Microarray Data Analysis: Part I
Lecture 36 : Microarray Data Analysis: Part II
Week 8:
Lecture 37 : Application of protein microarray in biomarker discovery-I
Lecture 38 : Application of protein microarray in biomarker discovery-II
Lecture 39 : Systems biology and networks
Lecture 40 : Challenges in proteomics
