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The objective of the course is to introduce methods used in NMR spectroscopy for structure determination of biomolecules and for studying protein-ligand interactions. The course will cover principles and application of two- and three-dimensional NMR experiments along with different isotope labelling schemes that are routinely used for protein structure determination.
INTENDED AUDIENCE:
M. Sc/Ph. D students, Scientists from Pharma/Biotech CompaniesPREREQUISITES: 1. Mathematics at the 12th Std level,2. Basic Knowledge of Proteins/Nucleic Acids,3. A basic knowledge of NMR would be useful,though not necessary
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Week 1 : Introduction to Basics of NMR
Week 2 : Two dimensional NMR
Week 3 : Important 2D NMR experiments for Biomolecules
Week 4 : 3D NMR Spectroscopy
Week 5 : Basics of Protein and Nucleic Acid Structure
Week 6 : Isotope Labelling
Week 7 : Resonance assignment of Proteins with NMR and Structure Determination
Week 8 : NMR Experiments for Studying Protein-Ligand Interactions