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Animal biotechnology is a branch of biotechnology in which molecular biology techniques are used to genetically engineer animals in order to improve their suitability for agriculture, industrial and pharmaceutical applications. Advances in animal biotechnology have been facilitated by recent progress in sequencing animal genomes, gene expression and metabolic profiling of animal cells. Genome editing technologies (Zinc Finger Nucleases, TALENS, and CRISPR-Cas systems) have opened up new opportunities to easily create genetic variations in animals that can improve their health and well-being, agricultural production, and protection against diseases. The objective of this course is to familiarize the techniques involved animal biotechnology. The course aims to provide theory and practical sessions of biotechnology as part of Professionalization. The course can be well utilized for students, teachers or public those who have aspiration to learn modern science which keeps the world today.
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Weeks 1
Day 1: Module 1- Introduction to Animal Biotechnology- Concept and Scope of BiotechnologyDay 2: Module 2 - Cloning vectors-I - Cosmids, Phagemids, Lambda Bacteriophage, M13, BAC, YAC, MACDay 3: Module 3 - Cloning vectors-II- Expression vectorsDay 4: Interaction\Discussion on Module 1, 2 & 3Day 5: Assignments
Weeks 2
Day 1: Module 4- Restriction enzymes IDay 2: Module 5 - Restriction enzymes types and detailed study of Type IIDay 3: Module 6 - Gene cloningDay 4: Interaction\Discussion on Module 4, 5 & 6Day 5: Assignments
Weeks 3
Day 1: Module 7- Gene transfer techniques
Day 2: Module 8 - Construction of genomic and cDNA librariesDay 3: Module 9 - screening of DNA LibrariesDay 4: Interaction\Discussion on Module 7, 8 & 9Day 5: Assignments
Weeks 4
Day 1: Module 10- Blotting techniques I (Southern and Northern)
Day 2: Module 11 - Blotting techniques II- Western blottingDay 3: Module 12 - DNA sequencingDay 4: Interaction\Discussion on Module 10, 11 & 12Day 5: Assignments
Weeks 5
Day 1: Module 13- Polymerase Chain ReactionDay 2: Module 14 - DNA Finger PrintingDay 3: Module 15 - DNA Micro array principle and technique.Day 4: Interaction\Discussion on Module 13, 14 & 15Day 5: Assignments
Weeks 6
Day 1: Module 16- Genetically modified organisms-I (Nuclear Transplantation)Day 2: Module 17 - Genetically modified organisms-II (Retroviral Method)Day 3: Module 18 - Genetically modified organisms-III (DNA microinjection)Day 4: Interaction\Discussion on Module 16, 17 & 18Day 5: Assignments
Weeks 7
Day 1: Module 19- Transgenic AnimalsDay 2: Module 20 - Applications of transgenic animals I- Production of PharmaceuticalsDay 3: Module 21- Applications of transgenic animals: production of donor organsDay 4: Interaction\Discussion on Module 19, 20 & 21Day 5: Assignments
Weeks 8
Day 1: Module 22- Applications of transgenic animals: knock out mice
Day 2: Module 23 - Production of transgenic plants- Agrobacterium mediated transformation
Day 3: Module 24 - Applications of transgenic plants: Insect and herbicide resistant plantsDay 4: Interaction\Discussion on Module 22, 23 & 24Day 5: Assignments
Weeks 9
Day 1: Module 25- Animal cell cultureDay 2: Module 26 - Expressing cloned genes in mammalian cellsDay 3: Module 27 - Diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia)Day 4: Interaction\Discussion on Module 25, 26 & 27Day 5: Assignments
Weeks 10Day 1: Module 28- Recombinant DNA in medicine I- Recombinant insulin & Human growth hormoneDay 2: Module 29 – Gene TherapyDay 3: Module 30 - Animal propagation-– Artificial inseminationDay 4: Interaction\Discussion on Module 28, 29 & 30Day 5: Assignments
Weeks 11
Day 1: Module 31- Conservation Biology I- In vitro fertilization and embryo transfer in humans
Day 2: Module 32 - Conservation Biology II- In vitro fertilization and embryo transfer in farm animalsDay 3: Module 33 - Animal diseases need help of Biotechnology- Foot-and-mouth diseaseDay 4: Interaction\Discussion on Module 31, 32 & 33Day 5: Assignments
Weeks 12Day 1: Module 34- Genomic DNA isolation from E. Coli-PracticalDay 2: Module 35 - Plasmid DNA isolation- PracticalDay 3: Module 36 - Restriction digestion- PracticalDay 4: Interaction\Discussion on Module 34, 35 & 36Day5: Term end assessment
